Principles of IVF Laboratory Practice Laboratory Set-Up, Training and Daily Operation

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• Chapters are short and follow a standard structure, with key references highlighted for further reading, allowing readers to absorb relevant information quickly
• Mirroring the life cycle of an IVF laboratory, chapters cover all aspects of set up from the first day of planning a clinic and establishing training protocols to quality control and troubleshooting once daily operations and practices are standardized
• Includes several chapters new to this edition expanding on developments in the field such as piezo-ICSI, safe and effective cryostorage and a chapter on managing emergency situations in the IVF laborator

The evolution of IVF practice
Section I. Starting a New Laboratory & Training Protocols:
1. Establishing and equipping a new IVF laboratory
2. Basic embryology skills and responsibilities in the IVF laboratory
3. Sperm preparation for IVF: training protocol
4. Oocyte pick-up for IVF: training protocol
5. Embryologist training: embryo transfer
6. ICSI for IVF: training protocol
7. Embryo assisted hatching for IVF: training protocol and method
8. Embryo biospy for IVF: training protocol
9. Vitrification for IVF: training protocol
10. Mouse embryo assay for quality control in the IVF laboratory
11. Sperm survival assay for quality control in the IVF laboratory
Section II. Pre-Procedure Protocols:
12. Quality control in the IVF laboratory: continuous improvement
13. Setup procedures for optimizing performance in the IVF laboratory
Section III. Gametes:
14. Sperm preparation for therapeutic IVF
15. Processing surgically retrieved sperm in the IVF laboratory
16. Cryopreservation of sperm for IVF: semen samples and individual sperm
17. Oocyte collection for IVF
18. In-vitro maturation of immature oocytes for IVM-IVF
19. Oocyte grading by morphological evaluation
20. Vitrification of oocytes for IVF
Section IV. Insemination/ICSI:
21. General insemination for IVF
22. Conventional IVF with short co-incubation
23. Technical aspects of ICSI for ejaculated spermatozoa
24. Set-up and adapted protocol for piezo-ICSI
25. Sperm selection for ICSI by morphology: MSOME/IMSI
26. Sperm selection for ICSI by viability
27. Artificial oocyte activation for IVF
Section V. Fertilization Assessmant:
28. Fertilization assessment in IVF and ICSI
29. Polar body biopsy for IVF
30. Zygote cryopreservation for IVF
Section VI. Embryo Assessment – Morphology & Beyond:
31. Embryo assessment at the pre-compaction stage in the IVF laboratory
32. Embryo assessment in the post-compaction stage in the IVF laboratory
33. Embryo assessment at the blastocyst stage in the IVF laboratory
34. Trophectoderm biopsy for preimplantation genetic testing
35. Embryo culture by Time-lapse: selection and beyond
Section VII. Embryo Cryopreservation:
36. Cryopreservation of pre-compaction embryos for IVF
37. Cryopreservation of post-compaction embryos for IVF
38. Safe and effective cryostorage of embryos for IVF
Section VIII. Embryo Transfer:
39. Routine embryo transfer in the IVF laboratory
Section IX. Quality Management:
40. Verification of specimen identity by double checking with a witness
41. Quality management in the IVF laboratory: quality improvement, document and process control and adverse events
42. Trouble-shooting in the IVF laboratory
43. Disaster preparedness in the IVF Laboratory.